Journal: NPJ Science of Food

Article Title: From rosemary and coffee to bioactive nanovesicles: exploring new frontiers in food functional ingredients

doi: 10.1038/s41538-026-00723-9

Figure Lengend Snippet: pAMPK ( A ), GLUT 4 ( B ), and FASN ( C ) protein levels, and quantification of intracellular lipid accumulation ( D ) in C2C12 cells pretreated with RVs and CVs 39 × 106 vesicles/mL in oxidative stress-induced damage. The data points represent the averages ±SD of three independent experiments in duplicate. All datasets were analyzed by one-way ANOVA followed by Tukey’s post hoc test. Different lowercase letters indicate a significant difference ( p 0.05) between different treatments. Control: untreated cells. RVs rosemary-derived vesicles, CVs coffee-derived vesicles, pAMPK AMP-activated protein kinase, GLUT-4 glucose transporter, FASN fatty acid synthase. 7.8 × 10 6 vesicles/mL and 39 × 10 6 vesicles/mL correspond to 0.1 and 0.5 mg nanovesicles/mL.

Article Snippet: The antibody against Collagen Type I (Cat No. 66761-1-Ig) and Fatty Acis Synthase (FASN, Cat No. 10624-2-AP), COX2/Cyclooxygenase 2 (Cat No. 12375-1-AP) and nuclear factor erythroid 2-related factor 2 (NRF2) (Cat No. 66504-1-Ig) were from Proteintech.

Techniques: Control, Derivative Assay

Journal: Cell Death Discovery

Article Title: Differential effects of HDAC inhibitors in the Rho I255d mouse model for autosomal dominant retinitis pigmentosa

doi: 10.1038/s41420-025-02908-9

Figure Lengend Snippet: Organotypic retinal explants were derived from wild-type (WT) and Rho I255d/+ mice at post-natal (P) day 12 and cultured with ACY-957 (ACY; A ) from P14 to P20. The non-treated (NT) WT situation was used as reference (dashed lines in B – D ). A The TUNEL assay (red) labeled dying cells in outer nuclear layer (ONL) and inner nuclear layer (INL) of both WT and Rho I255d/+ mutant retina. DAPI (grey) was used as nuclear counterstain. Quantification of TUNEL-positive cells in ONL ( B ) and INL ( C ). Note the strong rise in TUNEL positive cells at 10 µM ACY concentration. D In Rho I255d/+ mutant retina, the ONL row count indicated a significant increase at 10 µM ACY, when compared to NT mutant. Data represent mean and SD, and were obtained from 5 to 16 independent retinal explant cultures; statistical analysis: Two-way ANOVA test with two-stage linear step-up procedure of Benjamini, Krieger, and Yekutieli; * p ≤ 0.05, ** p ≤ 0.01, **** p ≤ 0.0001.

Article Snippet: ACY-957 (ACY, A) , , HDAC-1, , HDAC-1: 7 nM , MedChemExpress , , [ ] .

Techniques: Derivative Assay, Cell Culture, TUNEL Assay, Labeling, Mutagenesis, Concentration Assay

Journal: Cell Death Discovery

Article Title: Differential effects of HDAC inhibitors in the Rho I255d mouse model for autosomal dominant retinitis pigmentosa

doi: 10.1038/s41420-025-02908-9

Figure Lengend Snippet: Dose-response curves (log scale) for SAHA (magenta), MPT0G211 (green), and ACY-957 (blue). A Effects on cell death as evidenced by the TUNEL assay. B Photoreceptor survival as assessed by outer nuclear layer (ONL) row counts. C Numbers of cone photoreceptors per 100 µM of retinal circumference. D Quantification of HDAC activity positive cells in the ONL. E Quantification of ONL cells showing caspase-3 activation. F Quantification of ONL cells displaying calpain-2 activation. Data obtained from 4 to 16 independent retinal explant cultures; data points indicate mean and SD; statistical analysis: Two-way ANOVA test with two-stage linear step-up procedure of Benjamini, Krieger, and Yekutieli; * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.001, **** p ≤ 0.0001.

Article Snippet: ACY-957 (ACY, A) , , HDAC-1, , HDAC-1: 7 nM , MedChemExpress , , [ ] .

Techniques: TUNEL Assay, Activity Assay, Activation Assay